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- Title
Identification of a Novel Nucleus Protein Involved in the Regulation of Urokinase in 95D Cells.
- Authors
Tong, Chang; Tan, Li; Li, Ping; Zhu, Yun-Song
- Abstract
The urokinase-type plasminogen activator (uPA) plays an important role in cellular invasion. By using the downstream part of a 74 bp DNA region called the cooperation mediator (COM) of theuPApromoter as a bait sequence in the yeast one-hybrid screen, a gene calledPBK1was previously cloned from the cDNA library of the 95D lung cancer cell strain. In this study, the intracellular distribution of PBK1 was studied by using the transient transfection of pEGFP-C3-PBK1, and PBK1 was found to be localized in the nucleus. Co-transfection of pEGFP-C3-PBK1 and the deletion mutants of the pGL3-uPA promoter indicated that PBK1 can increase theuPApromoter activity by about 25% and this effect isuPAenhancer-dependent. Western blotting and Enzyme-linked immunoadsordent assay further confirmed that PBK1 can upregulate the expression ofuPA.Our results suggest that PBK1 is involved in the regulation ofuPAexpression, which might provide a new clue to further understanding the regulation mechanism ofuPAexpression.Edited byGary G. DENG
- Subjects
UROKINASE; CELLULAR immunity; PLASMINOGEN activators; PROTEOLYTIC enzymes; CIRCULAR DNA; GENE libraries
- Publication
Acta Biochimica et Biophysica Sinica, 2005, Vol 37, Issue 5, p303
- ISSN
1672-9145
- Publication type
Article
- DOI
10.1111/j.1745-7270.2005.00041.x