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- Title
Melanocyte spheroids are formed by repetitive long-term trypsinization.
- Authors
Li, Di; Zhang, Ru-Zhi; Shi, Hai-Xia; Yang, Yu-Hua; Tian, Ting; Wang, Li
- Abstract
<bold>Background: </bold>Autologous melanocyte transplantation plays an important role in the treatment of vitiligo.<bold>Objective: </bold>Previous studies have indicated that, compared with melanocytes growing in monolayers, melanocyte spheroids have a better survival in growth factor- and serum-deprived conditions.<bold>Methods: </bold>Melanocyte spheroids were obtained from human epidermis by repetitive long-term trypsinization and maintained an aggregated morphology for a short period in certain conditions.<bold>Results: </bold>Melanocyte spheroids were capable of growing into normal dendritic melanocytes in monolayer when they were harvested and reinoculated in 24-well plates. Immunohistochemical analysis of the melanocyte spheroids revealed that they were positive for HMB45, a melanosome-specific marker. No melanomas occurred when melanocyte spheroids were transplanted into mice.<bold>Conclusion: </bold>Our study provides a promising approach for melanocyte transplantation to treat vitiligo.
- Publication
Indian Journal of Dermatology, Venereology & Leprology, 2019, Vol 85, Issue 3, p258
- ISSN
0378-6323
- Publication type
journal article
- DOI
10.4103/ijdvl.IJDVL_275_17