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- Title
Differentiation of Mesenchymal Stem Cells into Neural Stem Cells Using Cerebrospinal Fluid.
- Authors
Ge, Wei; Ren, Chao; Duan, Xin; Geng, Deqin; Zhang, Caiyi; Liu, Xiaoyun; Chen, Hao; Wan, Meirong; Geng, Runlu
- Abstract
Optimization of a methodology for mesenchymal stem cells (MSCs) differentiation into neural stem cells (NSCs) using cerebrospinal fluid (CSF). MSCs were extracted from umbilical cord blood from healthy, full-term, newborn infants and from the bone marrow of patients. CSF was taken from healthy adult volunteers and patients. Four groups investigated were: A ( n = 8) cord blood MSC induced with healthy volunteer CSF (control group); B ( n = 7): patient MSCs induced with health volunteer CSF; Group C ( n = 12): patient MSCs induced with their own CSF; group D ( n = 6): cord blood MSCs induced with patient CSF. Following induction, cell differentiation state was examined using microscopy, flow cytometry, and immunohistochemistry. There were significantly more clinically applicable MSCs in Groups B and C than groups A and D ( P < 0.05) and Group B had significantly more clinically applicable MSCs than group C ( P < 0.05). The presence of NSCs was as with the MSCs. Group B had significantly more clinically applicable NSCs than all of the other groups. In addition, group B cells grew significantly faster than the other groups ( P < 0.05). Upon CSF induction, MSCs differentiated into NSCs suitable for clinical treatment. The source of the MSCs and/or CSF influenced the number of NSCs produced and the NSC growth rate. Thus, the source of MSCs and CSF should be considered before initiating a stem cell clinical treatment.
- Subjects
MESENCHYMAL stem cells; NEURAL stem cells; CEREBROSPINAL fluid; UMBILICAL cord; FLOW cytometry
- Publication
Cell Biochemistry & Biophysics, 2015, Vol 71, Issue 1, p449
- ISSN
1085-9195
- Publication type
Article
- DOI
10.1007/s12013-014-0222-z