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- Title
Cloning, Expression, and Bioinformatics Analysis of Heavy Metal Resistance Gene afe_1862 from Acidithiobacillus ferrooxidans L1 in Escherichia coli.
- Authors
Leng, Feifan; Li, Yuanli; Luo, Wen; Wei, Qingwei; Jing, Yanjun; Wang, Xiaoli; Yang, Mingjun; Wang, Yonggang
- Abstract
Molecular studies of copper and cadmium resistances in acidophilic bacteria are significant in biomining. In this study, afe_1862, which encodes a heavy metal-binding protein in Acidithiobacillus ferrooxidans L1, was amplified using PCR, cloned into the pET32a plasmid, and sequenced. Following SDS-PAGE analysis, optimization of the expression conditions and heterologous overexpression of afe_1862 in Escherichia coli BL21 in the presence of Cu2+ and Cd2+ were studied as well. The results indicated that AFE_1862 has higher resistance to Cu2+ than Cd2+. Bioinformatics analysis illustrated that AFE_1862 has a conserved HMA domain containing heavy metal-binding sites, which may play a role in transporting or detoxifying heavy metals.
- Subjects
THIOBACILLUS ferrooxidans; ANALYSIS of heavy metals; ESCHERICHIA coli; ACIDOPHILIC bacteria; BIOINFORMATICS
- Publication
Biological Trace Element Research, 2019, Vol 189, Issue 1, p291
- ISSN
0163-4984
- Publication type
Article
- DOI
10.1007/s12011-018-1462-3