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- Title
Intercellular Connections Related to Cell-Cell Crosstalk Specifically Recognized by an Aptamer.
- Authors
Zhang, Nan; Bing, Tao; Shen, Luyao; Song, Rusheng; Wang, Linlin; Liu, Xiangjun; Liu, Meirong; Li, Juan; Tan, Weihong; Shangguan, Dihua
- Abstract
Intercellular connections are an important pathway for cell-cell crosstalk. However, their formation mechanism and functions are far from being understood. The lack of molecular probes hampers the research in this area. Herein, we report a kind of intercellular connection that is specifically recognized by aptamer M17A2 generated by cell-SELEX against MCF-7R cells. These connections have different morphologies, but have the same skeleton composed of F-actin. The long filamentous connections were identified to be tunneling nanotubes (TNTs), a recently discovered cell-cell communication route. These connections could be built not only between MCF-7R cells, but also from MCF-7R to other cells after co-culture. Proteins could be transported between cells through these connections, suggesting their cell communication function. Aptamer M17A2 shows the potential to act as a new probe for investigating this kind of intercellular connection, as well as for studying cell-cell communication.
- Subjects
CELL communication; APTAMERS; PEPTIDES; SYNTHETIC antibodies; PROTEINS
- Publication
Angewandte Chemie, 2016, Vol 128, Issue 12, p3982
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201510786