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- Title
Optimising dental pulp stem cell adhesion on agarose scaffolds for in vitro dentinogenesis.
- Authors
Whitehouse, Laura; Do, Thuy; Thomson, Neil
- Abstract
Agarose is an attractive cellular scaffold material that could be used for dentine regeneration. It is bioinert, highly hydrated, porous and capable of being tuned to specific mechanical parameters. Owing to agarose's inert surface chemistry after gelation, it needs to be modified to facilitate cellular attachment. This paper summarises pilot work in exploring several means of overcoming the issues of cellular attachment to facilitate the creation of a dentinogenic scaffold based on agarose. Additions of laponite, gelatin, collagen and fibronectin were trialled as well as surface modification with polydopamine and poly-L-lysine being tested separately. Live/dead staining was completed with confocal microscopy and qualitatively assessed. The results suggest that poly-L-lysine is the best performing modification to the gel.
- Subjects
DENTAL pulp; CELL adhesion; AGAROSE; STEM cells; SURFACE chemistry
- Publication
Faculty Dental Journal, 2024, Vol 15, Issue 3, p112
- ISSN
2042-6852
- Publication type
Article
- DOI
10.1308/rcsfdj.2024.33