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- Title
Pyometra alters the redox status and expression of estrogen and progesterone receptors in the uterus of domestic cats.
- Authors
Nascimento, Acácia Eduarda de Jesus; Santos, Luciano Cardoso; Santos, Bianca Reis; Santos, Emilly Oliveira; Cunha, Maria Clara da Silva Galrão; Snoeck, Paola Pereira das Neves; de Lavor, Mário Sérgio Lima; Silva, Juneo Freitas
- Abstract
Objectives: The aim of this study was to evaluate the expression profile of sex steroid receptors and redox mediators in the uterus of domestic cats with pyometra. Methods: Twelve cats were used and divided into groups: (1) non-gestational healthy diestrus (n = 7) and (2) pyometra (n = 5). The plasma profiles of estradiol and progesterone (P4) as well as uterine expression levels of estradiol alpha (ERα), progesterone (PR) and androgen (AR) receptors, of the antioxidant enzymes superoxide dismutase 1 (SOD1), catalase and glutathione peroxidase 1 (GPX1), and of the oxidative damage marker 8-hydroxy-2′-deoxyguanosine (8-OHdG) were evaluated. Results: Cats with pyometra showed higher plasma P4 levels and increased uterine messenger RNA (mRNA) and protein expression of ERα and PR, mainly in the glandular epithelium for ERα and in stromal and myometrial cells for PR. In addition, there was an increase in 8-OHdG immunostaining and GPX1 mRNA and protein expression in cats with pyometra compared with those in non-gestational diestrus, while catalase showed a reduction in endometrial immunostaining in cats with pyometra. There were no differences in uterine AR and SOD1 expression between the groups. Conclusion and relevance: The findings of this study showed that pyometra is associated with oxidative stress in the uterus of domestic cats and alterations of the profile of sex steroid receptors, especially ERα and PR, and of antioxidant enzymes, suggesting that changes in these mediators may play a role with the etiopathogenesis of this disease.
- Publication
Journal of Feline Medicine & Surgery, 2023, Vol 25, Issue 11, p1
- ISSN
1098-612X
- Publication type
Article
- DOI
10.1177/1098612X231170159