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- Title
Correction to: MicroRNA‑148a‑3p alleviates high glucose‑induced diabetic retinopathy by targeting TGFB2 and FGF2.
- Authors
Wang, Jihong; Yao, Yong; Wang, Kelei; Li, Jia; Chu, Ting; Shen, Haicui
- Abstract
1 MiR-148a-3p level is reduced by HG treatment. a TUNEL assay was used to measure cell apoptosis induced by different concentration of high glucose for 48 h. b Cell viability of HREC cells in the context of different concentration of high glucose (48 h) was assessed by CCK-8 assay. c The level of miR-148a-3p in HREC cells induced by different concentration of high glucose for 48 h. *P < 0.05, **P < 0.01 Graph: Fig. 2 Overexpression of miR-148a-3p suppresses the cell apoptosis and BRB injury. a The overexpression efficiency of miR-148a-3p mimics was determined by RT-qPCR analysis 24 h after plasmid transfection. b CCK-8 assay was utilized to measure cell viability 24 h after plasmids transfection. c Cell apoptosis in different groups was detected by TUNEL assay 24 h after plasmid transfection. d Western blot analysis was adopted to test claudin 3, occludin and ZO-1 protein levels 24 h after plasmids transfection. e Tube formation of HRECs in different groups 24 h after plasmids transfection. 4 MiR-148a-3p alleviates BRB injury by targeting TGFB2. a The overexpression efficacy of pcDNA3.1/TGFB2 was assessed by western blot analysis 24 h after plasmids transfection. b CCK-8 assay was utilized to measure cell viability in different groups 24 h after plasmids transfection. c Cell apoptosis in different groups was detected by TUNEL assay 24 h after plasmids transfection. d Western blot analysis was adopted to test claudin 3, occludin and ZO-1 protein levels 24 h after plasmids transfection.
- Subjects
DIABETIC retinopathy; OCCLUDINS
- Publication
Acta Diabetologica, 2023, Vol 60, Issue 1, p147
- ISSN
0940-5429
- Publication type
Article
- DOI
10.1007/s00592-022-01997-7