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- Title
Well-oriented bioarchitecture for immobilization of chloroperoxidase on graphene oxide nanosheets by site-specific interactions and its catalytic performance.
- Authors
Ding, Yu; Cui, Ru; Hu, Mancheng; Li, Shuni; Zhai, Quanguo; Jiang, Yucheng
- Abstract
Chloroperoxidase (CPO) was immobilized on graphene oxide (GO) nanosheets via site-specific interactions of concanavalin (ConA) with saccharide groups on the CPO surface to make GO-ConA-CPO nano-architectonics. Enzymatic oxidative decolorization of malachite green was used to investigate the catalytic performance of GO-ConA-CPO. The GO-ConA-CPO showed high activity (based on the decolorization efficiency), i.e., 93.68% in 15 min. Moreover, GO-ConA-CPO showed better thermostability and remained higher activity against pH change and high concentrations of oxidant HO compared with GO-CPO and the free enzyme. When incubated at 60 °C for 1.5 h, 63.02% of the activity of GO-ConA-CPO and 35.75% of GO-CPO remained compared with that at 25 °C, while free CPO remained only 8.46% in the same condition. The tolerance of GO-ConA-CPO to HO improved from 2.5 to 6 mmol L, and the suitable pH range enlarged from 2.5-3.0 to 2.0-4.5. After 8 cycles, the GO-ConA-CPO can keep 52% activity of that in the first run. The enzymatic kinetic constants indicated that introducing CPO into GO-ConA-CPO bioarchitecture improved the diffusion of reactants and products.
- Subjects
GRAPHENE oxide; CARBON nanotubes; CATALYTIC activity; CHLOROPEROXIDASE; MALACHITE
- Publication
Journal of Materials Science, 2017, Vol 52, Issue 17, p10001
- ISSN
0022-2461
- Publication type
Article
- DOI
10.1007/s10853-017-1202-7