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- Title
Synthesis of vanillic acid using whole cell nitrilase of wild and mutant Gordonia terrae.
- Authors
Bhalla, Tek; Prashant; Kumari, Nisha; Kumar, Vijay; Kumar, Virender; Savitri
- Abstract
The resting cells of Gordonia terrae mutant E9 having enhanced nitrilase activity were used for biotransformation of 4-hydroxy-3-methoxybenzonitrile into vanillic acid. The maximum conversion was observed in 0.1 M phosphate buffer (pH 8.0), using 60 mM substrate and 0.75 mg resting cells in 1 mL reaction at 40 °C. K of the whole cell nitrilase of wild and mutant strains of G. terrae for this substrate were 20 and 16.6 mM, and V were 0.19 and 0.95 $${\text{Umg}}_{{_{\text{DCW}} }}^{{^{ - 1} }}$$ , respectively. Fed batch reaction for transformation of 4-hydroxy-3-methoxybenzonitrile using whole cell nitrilase of wild G. terrae resulted in 2.36 g of vanillic acid in 5 h with a catalytic and volumetric productivity of 0.78 $${\text{gg}}_{\text{DCW}}^{ - 1} {\text{h}}^{ - 1}$$ and 4.72 gLh, respectively. The whole cell nitrilase of G. terrae mutant E9 resulted in higher catalytic and volumetric productivity, i.e., 1.68 $${\text{gg}}_{\text{DCW}}^{ - 1} {\text{h}}^{ - 1}$$ and 10 gLh. A total 5.04 g of vanillic acid with 99 % purity were accumulated in 100 mL of reaction after 5 h.
- Subjects
NITRILASES; CELL culture; CHEMICAL synthesis; GENETIC mutation; BIOTRANSFORMATION (Metabolism)
- Publication
Bioprocess & Biosystems Engineering, 2016, Vol 39, Issue 1, p67
- ISSN
1615-7591
- Publication type
Article
- DOI
10.1007/s00449-015-1490-8