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- Title
Use of Gene Expression Analysis for Discrimination of Primary and Secondary Adenocarcinoma of the Liver.
- Authors
Hass, Holger G.; Vogel, Ulrich; Scheurlen, Michael; Jobst, Jürgen
- Abstract
Background: Due to late diagnosis and resistance to chemotherapy, most patients with cholangiocarcinoma have an unfavorable prognosis. Despite the use of immunohistochemistry (IHC) in clinical routine, differentiation between intrahepatic cholangiocarcinoma (ICC) and secondary adenocarcinomas of the liver is frequently not clear, leading to false diagnosis and treatment decisions. Methods: Oligonucleotide microarrays (Affymetrix Hu133A©) were used for gene expression analysis of ICC (n = 11) and secondary adenocarcinomas (colorectal metastases; n = 6). By two-dimensional cluster analysis a specific gene expression profile of these tumors was established and confirmed by real-time polymerase chain reaction and IHC. Results: A total of 338 genes were significantly dysregulated (gene expression/fc ≥2; dysregulation in ≥60%) in both tumor groups. Using two-dimensional cluster analysis a fast, clear, and reproducible differentiation between ICC and colorectal metastases was possible in all cases. As potential biomarkers for differentiation, twelve genes (ICC: KRT7, DBN1, LCTB, LIF, STK17A, PIGF; metastases: TDGF1, HOXA9, TFF3, MYB, ABP1, BCL11A) were detected and will be used for further investigations. Conclusions: A specific gene expression profile for discrimination of primary and secondary adenocarcinoma of the liver could be established. In addition, marker genes for both cancers and their potential use as discrimination markers in clinical routine were also described partially for the first time.
- Subjects
ADENOCARCINOMA; CHOLANGIOCARCINOMA; LIVER tumors; CLUSTER analysis (Statistics); COLON tumors; GENE expression; METASTASIS; ONCOGENES; POLYMERASE chain reaction; RECTUM tumors; TUMOR markers; TUMOR classification; OLIGONUCLEOTIDE arrays; GENE expression profiling; DIAGNOSIS
- Publication
Oncology, 2018, Vol 95, Issue 4, p211
- ISSN
0030-2414
- Publication type
Article
- DOI
10.1159/000489563