We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Altered expression of KCNK9 in colorectal cancers.
- Authors
Chang AJe Kim; Yong Gu Cho; Seong Whan Jeong; Young Sil Kim; Su Young Kim; Suk Woo Nam; Sug Hyung Lee; Nam Jin Yoo; Jung Young Lee; Won Sang Park
- Abstract
Kim CJ, Cho YG, Jeong SW, Kim YS, Kim SY, Nam SW, Lee SH, Yoo NJ, Lee JY, Park WS. Altered expression of KCNK9 in colorectal cancer. APMIS 2004;112:588–94.K+ channels have been reported to be involved in the proliferation of many types of cells, including some human carcinoma and tumor cell lines. KCNK9, a TASK channel, is amplified and overexpressed in several types of human cancer. In the present study, we examined the expression and somatic mutations of KCNK9 in 124 colorectal cancers by immunohistochemistry using tissue microarray and PCR-SSCP. Immunopositivity was observed in 57 (46.0%) of 124 colorectal cancers. Clinically, KCNK9 was immunopositive in 4 (30.7%) of 13 cases which were stage A, 26 (55.3%) of 47 which were stage B, 23 (41.1%) of 56 which were stage C, and 4 (50%) of 8 which were stage D. Statistically, KCNK9 protein expression was not related to tumor stage (Bartholomew test, p>0.05) and lymph node metastasis (Chi-Square test, p=0.8338). In the mutation study of theKCNK9gene, we found only one sequence variation (ACG→ACC, Thr→Thr) at codon 170 both in corresponding normal and tumor DNAs. These results indicate that overexpression rather than mutation of theKCNK9gene may contribute to the development of colorectal cancers and suggest that the development of KCNK9-targeted agents may provide new possibilities in the treatment of colorectal cancer.
- Subjects
GENE expression; COLON cancer; IMMUNOHISTOCHEMISTRY; LYMPH nodes; GENETIC mutation
- Publication
APMIS, 2004, Vol 112, Issue 9, p588
- ISSN
0903-4641
- Publication type
Article
- DOI
10.1111/j.1600-0463.2004.apm1120905.x