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- Title
A novel method for the detection of pyrrolizidine alkaloids in bottled tea and tea leaves by LC-MS/MS.
- Authors
Yoshifumi Takatsuji; Ayano Kakitani; Yasushi Nagatomi; Koichi Harayama; Koji Suzuki
- Abstract
Pyrrolizidine alkaloids are products of secondary metabolism in plants. They have been detected in honey, as well as in herbal and green tea. However, these molecules are toxic and carcinogenic, and hence, their presence in commercial products should be regulated. In this study, a quantitative analytical method for the detection of 19 pyrrolizidine alkaloid compounds in bottled tea and tea leaves was developed. The samples were prepared via the QuEChERS method and the analysis was performed with a tandem of high performance liquid chromatography and mass spectrometry. Monitoring tests were conducted on commercially available bottled tea and tea leaves. The results showed that in Japanese commercial bottled tea, all pyrrolizidine alkaloids were below the detection limit. Tea leaves experiments, in contrast, revealed that some types of tea (rooibos and chamomile) contain pyrrolizidine alkaloids.
- Subjects
PYRROLIZIDINES; SECONDARY metabolism; GREEN tea; CARCINOGENICITY; COMMERCIAL products; HIGH performance liquid chromatography
- Publication
Japanese Journal of Food Chemistry & Safety, 2018, Vol 25, Issue 2, p97
- ISSN
1341-2094
- Publication type
Article