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- Title
Microbiological Methods Used in the Enterics for Global Health Shigella Surveillance Study.
- Authors
Horne, Bri'Anna; Badji, Henry; Bhuiyan, Md Taufiqur Rahman; Cachique, Lucero Romaina; Cornick, Jennifer; Hotwani, Aneeta; Juma, Jane; Ochieng, John Benjamin; Abdou, Mahamadou; Apondi, Evans; Atlas, Hannah E; Awuor, Alex O; Baker, Kate S; Ceesay, Bubacarr E; Charles, Mary; Cunliffe, Nigel A; Feutz, Erika; Galagan, Sean R; Guindo, Ibrehima; Hossain, M Jahangir
- Abstract
Background Shigella is a major cause of diarrhea in young children worldwide. Multiple vaccines targeting Shigella are in development, and phase 3 clinical trials are imminent to determine efficacy against shigellosis. Methods The Enterics for Global Health (EFGH) Shigella surveillance study is designed to determine the incidence of medically attended shigellosis in 6- to 35-month-old children in 7 resource-limited settings. Here, we describe the microbiological methods used to isolate and identify Shigella. We developed a standardized laboratory protocol for isolation and identification of Shigella by culture. This protocol was implemented across all 7 sites, ensuring consistency and comparability of results. Secondary objectives of the study are to determine the antibiotic resistance profiles of Shigella , compare isolation of Shigella from rectal swabs versus whole stool, and compare isolation of Shigella following transport of rectal swabs in Cary-Blair versus a modified buffered glycerol saline transport medium. Conclusions Data generated from EFGH using culture methods described herein can potentially be used for microbiological endpoints in future phase 3 clinical trials to evaluate vaccines against shigellosis and for other clinical and public health studies focused on these organisms.
- Subjects
SHIGELLA; VACCINE trials; CLINICAL trials; WORLD health; RESOURCE-limited settings
- Publication
Open Forum Infectious Diseases, 2024, Vol 11, pS25
- ISSN
2328-8957
- Publication type
Article
- DOI
10.1093/ofid/ofad576