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- Title
Parasitemia and Differential Tissue Tropism in Mice Infected with Trypanosoma cruzi Isolates Obtained from Meccus phyllosoma in the State of Oaxaca, Mexico.
- Authors
Flores-Villegas, Any Laura; Jiménez-Cortés, Jesús Guillermo; González, James; Moreno-Rodríguez, Adriana; Pérez-Cabeza de Vaca, Rebeca; Segal-Kischinevzky, Claudia; Bucio-Torres, Martha I.; De Fuentes-Vicente, José A.; Nava-Lazaro, Elisabeth; Salazar-Schettino, Paz María; Cabrera Bravo, Margarita
- Abstract
Trypanosoma cruzi is a parasite transmitted by the feces of triatomines. Many triatomine species are found in Mexico, and various T. cruzi variants have been isolated from these species, each showing very different virulence and cell tropism. The isolates were obtained from Meccus phyllosoma specimens in three localities in the state of Oaxaca, Mexico: Tehuantitla, Vixhana, and Guichivere. The virulence of each isolate was assessed by quantifying parasitemia, survival, and histopathologic findings. The lineage of each isolate was identified using the mini-exon gene. The expression of the tssa gene during infection was detected in the heart, esophagus, gastrocnemius, and brain. Our results show that the maximum post-infection parasitemia was higher for the Tehuantitla isolate. On genotyping, all isolates were identified as T. cruzi I. The amastigotes in the heart and gastrocnemius were verified for all isolates, but in the brain only for Tehuantitla and Vixhana. The tssa expression allowed us to detect T. cruzi isolates, for Tehuantitla, predominantly in the heart. For Vixhana, a higher tssa expression was detected in gastrocnemius, and for Guichivere, it was higher in the esophagus. Results show that virulence, tropism, and tssa expression can vary, even when the isolates are derived from the same vector species, in the same region, and at similar altitudes.
- Subjects
OAXACA (Mexico : State); MEXICO; TRYPANOSOMA cruzi; VIRAL tropism; PARASITEMIA; TROPISMS; CONENOSES; TISSUES
- Publication
Pathogens, 2022, Vol 11, Issue 10, p1141
- ISSN
2076-0817
- Publication type
Article
- DOI
10.3390/pathogens11101141