We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency.
- Authors
Umair, Zobia; Baek, Mi-Ock; Song, Jisue; An, Seona; Chon, Seung Joo; Yoon, Mee-Sup
- Abstract
Premature ovarian insufficiency (POI) is a typical disorder of amenorrhea that lasts for a minimum of four months in women < 40 years old and is typically characterized by reduced estrogen levels and elevated serum concentrations of follicle-stimulating hormone. We collected urine samples from two participant cohorts from Gil Hospital of Gachon University (Incheon, Korea): a sequencing cohort of 19 participants (seven patients with POI (POI patients without Turner syndrome), seven patients with Turner syndrome (POI patients with Turner syndrome), and five control individuals (age-matched controls with confirmed ovarian sufficiency)) and a validation cohort of 46 participants (15 patients with POI, 11 patients with Turner syndrome, and 20 control individuals). Among differentially expressed miRNAs, hsa-miR-4516 was significantly upregulated in patients with POI in both cohorts, independent of the presence of Turner syndrome. Moreover, the upregulation of miR-4516 was confirmed in the ovary—but not in the uterus—of a cyclophosphamide and busulfan-induced POI mouse model. This was accompanied by a decrease in STAT3 protein level, a predicted target of miR-4516, via miRTarBase2020. Our study provides compelling evidence that miR-4516 is highly expressed in patients with POI and POI mouse models, suggesting that miR-4516 is a diagnostic marker of POI.
- Subjects
PREMATURE ovarian failure; EXOSOMES; TURNER'S syndrome; BIOMARKERS; FOLLICLE-stimulating hormone; UNIVERSITY hospitals
- Publication
Cells (2073-4409), 2022, Vol 11, Issue 18, pN.PAG
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells11182797