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- Title
Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime.
- Authors
Krenkel, Martin; Toepperwien, Mareike; Alves, Frauke; Salditt, Tim
- Abstract
X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources provided by X-ray waveguides, here this approach is extended in several ways. First, the phase-retrieval algorithms are extended by an optimized deterministic inversion, based on a multi-distance recording. Second, different advanced forms of iterative phase retrieval are used, operational for single-distance and multi-distance recordings. Results are compared for several different preparations of macrophage cells, for different staining and labelling. As a result, it is shown that phase retrieval is no longer a bottleneck for holographic imaging of cells, and how advanced schemes can be implemented to cope also with high noise and inconsistencies in the data.
- Subjects
X-ray holography; THEORY of wave motion; WAVE mechanics
- Publication
Acta Crystallographica. Section A, Foundations & Advances, 2017, Vol 73, Issue 4, p282
- ISSN
2053-2733
- Publication type
Article
- DOI
10.1107/S2053273317007902