We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A Gain-of-Function Screen Identifying Genes Required for Growth and Pattern Formation of the Drosophila melanogaster Wing.
- Authors
Cruz, Cristina; Glavic, Alvaro; Casado, Mar; de CeIis, Jose F.
- Abstract
The Drosophila melanogaster wing is a model system for analyzing the genetic control of organ size, shape, and pattern formation. The formation of the wing involves a variety of processes, such as cell growth, proliferation, pattern formation, and differentiation. These developmental processes are tinder genetic control, and many genes participating in specific aspects of wing development have already being characterized. In this work, we aim to identify novel genes regulating wing growth and patterning. To this end, we have carried out a gain-of-function screen generating novel P-UAS (upstream activating sequences) insertions allowing forced gene expression. We produced 3340 novel P-UAS insertions and isolated 300 that cause a variety of wing phenotypes in combination with a Ga14 driver expressed exclusively in the central domain of the presumptive wing blade. The mapping of these P-UAS insertion sites allowed us to identify the gene that causes the gain-of-function phenotypes. We show that a fraction of these phenotypes are related to the induction of cell death in the domain of ectopic gene expression. Finally, we present a preliminary characterization of a gene identified in the screen, the function of which is required for the development of the L5 longitudinal vein.
- Subjects
GENES; DROSOPHILA melanogaster; GENOTYPE-environment interaction; GENETIC regulation; CELL death; CELL proliferation
- Publication
Genetics, 2009, Vol 183, Issue 3, p1005
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.109.107748