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- Title
Exploring capabilities of elemental mass spectrometry for determination of metal and biomolecules in extracellular vesicles.
- Authors
Martínez-García, Jaime; Villa-Vázquez, Alicia; Fernández, Beatriz; González-Iglesias, Héctor; Pereiro, Rosario
- Abstract
Extracellular vesicles (EVs) are increasingly recognized as crucial components influencing various pathophysiological processes, such as cellular homeostasis, cancer progression, and neurological disease. However, the lack of standardized methods for EV isolation and classification, coupled with ambiguity in biochemical markers associated with EV subtypes, remains a major challenge. This Trends article highlights the most common approaches for EV isolation and characterization, along with recent applications of elemental mass spectrometry (MS) to analyse metals and biomolecules in EVs obtained from biofluids or in vitro cellular models. Considering the promising capabilities of elemental MS, the article also looks ahead to the potential analysis of EVs at the single-vesicle and single-cell levels using ICP-MS. These approaches may offer valuable insights into individual characteristics of EVs and their functions, contributing to a deeper understanding of their role in various biological processes.
- Subjects
EXTRACELLULAR vesicles; MASS spectrometry; BIOMOLECULES; BIOMARKERS; CHARACTERISTIC functions
- Publication
Analytical & Bioanalytical Chemistry, 2024, Vol 416, Issue 11, p2595
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-023-05056-0