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- Title
Initiation of adult myelopoiesis can occur in the absence of c-Myb whereas subsequent development is strictly dependent on the transcription factor.
- Authors
Sumner, Robert; Crawford, Anthony; Mucenski, Michael; Frampton, Jonathan
- Abstract
The c-Myb transcriptional regulator is crucial to the development and functioning of haemopoietic cells, so much so that mouse embryos homozygous for an inactivated c-myb allele die from anaemia at about day 15 of gestation. By analysing c-myb-/- chimaeras we show that no mature cells of any lymphoid or myeloid lineage can be detected in adult haemopoietic tissues. This demonstrates that the effects of c-myb ablation on haemopoiesis are cell autonomous and correlates with an absence in the c-myb-/- foetal liver of uni- and multi-lineage CFUs. Indeed, CFU assays performed on E8.5 yolk sac cells revealed that haemopoietic progenitors are already defective at this stage. However, although cells expressing high levels of c-Kit were absent, we could detect a high proportion of CD34+CD45+ cells in the c-myb-/- foetal liver. Examination of chimaeric embryos revealed that c-myb-/- donor-derived CD34+/Kit+ cells, representing committed definitive progenitors, initially populated the foetal liver, but are unable to expand like wild type progenitors. Our results showing no megakaryocytic CFUs and a reduction in the absolute numbers of megakaryocytes in the c-myb-/- foetal liver also refute early suggestions that megakaryopoiesis is unaffected by the absence of c-Myb. Oncogene (2000) 19, 3335–3342
- Subjects
TRANSCRIPTION factors; HEMATOPOIETIC growth factors; FETAL liver cells; MEGAKARYOCYTES
- Publication
Oncogene, 2000, Vol 19, Issue 30, p3335
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1203660