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- Title
Mouse fibroblast growth factor 10: cDNA cloning, protein characterization, and regulation of mRNA expression.
- Authors
Beer, Hans-Dietmar; Florence, Charles; Dammeier, Johanna; McGuire, Linda; Werner, Sabine; Duan, D Roxanne
- Abstract
Fibroblast growth factor 7 (FGF-7) or keratinocyte growth factor (KGF), is a potent and specific mitogen for epithelial cells. We have recently identified a novel human FGF-7 homologue, named FGF-10.To study the expression of this new FGF family member and its regulation in wound repair, we cloned the mouse FGF-10 (mFGF-10) cDNA. The encoded protein is 92% identical to human FGF-10 and 91% identical to rat FGF-10. When expressed in mammalian 293 cells, the mFGF-10 protein was glycosylated but remained cell- or extracellular matrix-associated. Upon addition of heparin, mFGF-10 protein was released into the media. mRNA encoding mFGF-10 was relatively abundant in lung, skin, brain and heart. In the skin, both FGF-7 and mFGF-10 were expressed in the dermal, but not the epidermal compartment. In contrast to FGF-7, mFGF-10 expression was not induced during cutaneous wound repair. In cultured fibroblasts, expression of mFGF-10 was strongly repressed by transforming growth factor β and tumor necrosis factor α, whereas epidermal growth factor and interleukin-1β had no effect. These results demonstrate a differential regulation of mFGF-10 and FGF-7 expression in vitro and during the wound healing process.
- Subjects
FIBROBLAST growth factors; MOLECULAR cloning; GENETIC regulation; MESSENGER RNA; GENE expression
- Publication
Oncogene, 1997, Vol 15, Issue 18, p2211
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1201383