We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Biotransformation of the Proteogenic Amino Acids Phenylalanine, Tyrosine and Tryptophan by Yarrowia Species: An Application to the Preparative Synthesis of Natural Phenylacetic Acid.
- Authors
Serra, Stefano; Castagna, Antonio; Marzorati, Stefano; Valentino, Mattia
- Abstract
The biotransformation of the aromatic amino acids phenylalanine, tyrosine and tryptophan originates a number of bioactive compounds. Yeasts are the most used microorganisms for the transformation of (L)-phenylalanine into the flavour phenylethanol. Here, we reported a study on the biotransformation of the proteogenic aminoacids phenylalanine, tyrosine and tryptophan by yeast strains belonging to Yarrowia genus. We found that the latter microorganisms, in high aerobic conditions, metabolise the aromatic amino acids (L)-phenylalanine and (L)-tyrosine with the almost exclusive formation of phenylacetic acid and 4-hydroxyphenylacetic acid, respectively. Differently, the biotransformation of (L)-tryptophan with Y. lipolytica, gave anthranilic acid as the main product. As stated by the European and USA legislations concerning natural flavour production, phenylacetic acid obtained by microbial conversion of phenylalanine of natural origin can be commercialised as a natural flavour. Accordingly, our findings were exploited in a new process, based on the Yarrowia strains-mediated biotransformation of natural (L)-phenylalanine, that allows the large-scale preparation of the high-value, natural flavour, phenylacetic acid.
- Subjects
PHENYLACETIC acid; AMINO acids; AMINOBENZOIC acids; BIOCONVERSION; TYROSINE; TRYPTOPHAN; PHENYLALANINE
- Publication
Catalysts (2073-4344), 2022, Vol 12, Issue 12, p1638
- ISSN
2073-4344
- Publication type
Article
- DOI
10.3390/catal12121638