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- Title
Effect of folA gene in human breast milk-derived Limosilactobacillus reuteri on its folate biosynthesis.
- Authors
Yu Jiang; Xianping Li; Wei Zhang; Yadong Ji; Kai Yang; Lu Liu; Minghui Zhang; Weicang Qiao; Junying Zhao; Mengjing Du; Xiaofei Fan; Xingfen Dang; Huo Chen; Tiemin Jiang; Lijun Chen
- Abstract
Introduction: Folate supplementation is crucial for the human body, and the chemically synthesized folic acid might have undesirable side effects. The use of molecular breeding methods to modify the genes related to the biosynthesis of folate by probiotics to increase folate production is currently a focus of research. Methods: In this study, the folate-producing strain of Limosilactobacillus reuteri B1-28 was isolated from human breast milk, and the difference between B1-28 and folA gene deletion strain AFolA was investigated by phenotyping, in vitro probiotic evaluation, metabolism and transcriptome analysis. Results: The results showed that the folate producted by the AFolA was 2-3 folds that of the B1-28. Scanning electron microscope showed that AFolA had rougher surface, and the acid-producing capacity (p = 0.0008) and adhesion properties (p = 0.0096) were significantly enhanced than B1-28. Transcriptomic analysis revealed that differentially expressed genes were mainly involved in three pathways, among which the biosynthesis of ribosome and aminoacyl-tRNA occurred in the key metabolic pathways. Metabolomics analysis showed that folA affected 5 metabolic pathways, involving 89 different metabolites. Discussion: In conclusion, the editing of a key gene of folA in folate biosynthesis pathway provides a feasible pathway to improve folate biosynthesis in breast milk-derived probiotics.
- Subjects
FOLIC acid; PROBIOTICS; BIOSYNTHESIS; HUMAN genes; SCANNING electron microscopes; DELETION mutation; BREAST milk
- Publication
Frontiers in Microbiology, 2024, p01
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2024.1402654