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- Title
A Natural Dietary Supplement with a Combination of Nutrients Prevents Neurodegeneration Induced by a High Fat Diet in Mice.
- Authors
Nuzzo, Domenico; Picone, Pasquale; Galizzi, Giacoma; Di Carlo, Marta; Amato, Antonella; Terzo, Simona; Mulè, Flavia; Bonina, Francesco Paolo
- Abstract
Obesity and metabolic disorders can be risk factors for the onset and development of neurodegenerative diseases. The aim of the present study was to investigate the protective effects of a natural dietary supplement (NDS), containing Curcuma longa, silymarin, guggul, chlorogenic acid and inulin, on dysmetabolism and neurodegeneration in the brains of high fat diet (HFD)-fed mice. Decrease in the expression of FACL-4, CerS-1, CerS-4, cholesterol concentration and increase in the insulin receptor expression and insulin signaling activation, were found in brains of NDS-treated HFD brains in comparison with HFD untreated-mice, suggesting that NDS is able to prevent brain lipid accumulation and central insulin resistance. In the brains of NDS-treated HFD mice, the levels of RNS, ROS and lipid peroxidation, the expression of p-ERK, H-Oxy, i-NOS, HSP60, NF-kB, GFAP, IL-1β, IL-6 and CD4 positive cell infiltration were lower than in untreated HFD mice, suggesting antioxidant and anti-inflammatory effects of NDS. The decreased expression of p-ERK and GFAP in NDS-treated HFD mice was confirmed by immunofluorescence. Lastly, a lower number of apoptotic nuclei was found in cortical sections of NDS-treated HFD mice. The present data indicate that NDS exerts neuroprotective effects in HFD mice by reducing brain fat accumulation, oxidative stress and inflammation and improving brain insulin resistance.
- Subjects
BRAIN diseases; INSULIN resistance; PREVENTION of obesity; NEURODEGENERATION; INFLAMMATION prevention; HYPERLIPIDEMIA; REACTIVE oxygen species; ANIMAL experimentation; ANTI-inflammatory agents; ANTIOXIDANTS; CELL receptors; CELLULAR signal transduction; CHOLESTEROL; CYTOSKELETAL proteins; DIETARY supplements; FATTY acids; FLAVONOIDS; FLUORESCENT antibody technique; FAT content of food; GENE expression; GLUCANS; INSULIN; INTERLEUKINS; LIPID peroxidation (Biology); MICE; NERVOUS system; NUTRITIONAL requirements; OXIDOREDUCTASES; PROTEINS; TRANSFERASES; CD4 antigen; DNA-binding proteins; PLANT extracts; OXIDATIVE stress; NEUROPROTECTIVE agents; CARBOCYCLIC acids; PHARMACODYNAMICS; PREVENTION
- Publication
Nutrients, 2018, Vol 10, Issue 9, p1130
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu10091130