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- Title
Difference in transient ischemia-induced neuronal damage and glucose transporter-1 immunoreactivity in the hippocampus between adult and young gerbils.
- Authors
Seung Min Park; Jae-Chul Lee; Bai Hui Chen; Bich-Na Shin; Jeong Hwi Cho; In Hye Kim; Joon Ha Park; Moo-Ho Won; Ji Hyeon Ahn; Hyun-Jin Tae; Myoung Cheol Shin; Chan Woo Park; Jun Hwi Cho; Hui Young Lee
- Abstract
Objective(s): The alteration of glucose transporters is closely related with the pathogenesis of brain edema. We compared neuronal damage/death in the hippocampus between adult and young gerbils following transient cerebral ischemia/reperfusion and changes of glucose transporter-1(GLUT-1)-immunoreactive microvessels in their ischemic hippocampal CA1 region. Materials and Methods: Transient cerebral ischemia was developed by 5-min occlusion of both common carotid arteries. Neuronal damage was examined by cresyl violet staining, NeuN immunohistochemistry and Fluoro-Jade B histofluorescence staining and changes in GLUT-1 expression was carried out by immunohistochemistry. Results: About 90% of pyramidal neurons only in the adult CA1 region were damaged after ischemia/reperfusion; in the young, about 53 % of pyramidal neurons were damaged from 7 days after ischemia/reperfusion. The density of GLUT-1-immunoreactive microvessels was significantly higher in the young sham-group than that in the adult sham-group. In the ischemia-operatedgroups, the density of GLUT-1-immunoreactive microvessels was significantly decreased in the adult and young at 1 and 4 days post-ischemia, respectively, thereafter, the density of GLUT-1-immunoreactive microvessels was gradually increased in both groups after ischemia/reperfusion. Conclusion: CA1 pyramidal neurons of the young gerbil were damaged much later than that in the adult and that GLUT-1-immunoreactive microvessels were significantly decreased later in the young. These data indicate that GLUT-1 might differently contribute to neuronal damage according to age after ischemic insults.
- Subjects
GLUCOSE transporters; REPERFUSION injury; PYRAMIDAL neurons; HIPPOCAMPUS diseases; GERBILS as laboratory animals
- Publication
Iranian Journal of Basic Medical Sciences, 2016, Vol 19, Issue 5, p821
- ISSN
2008-3866
- Publication type
Article