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- Title
Characterization of a thermophilic and halotolerant Geobacillus pallidus H9 and its application in microbial enhanced oil recovery (MEOR).
- Authors
Wenjie, Xia; Li, Yu; Ping, Wang; Jianlong, Xiu; Hanping, Dong
- Abstract
A strain isolated from the oil reservoir in northern China was identified as Geobacillus pallidus by 16S rRNA gene sequencing. It could grow at a temperature of 45-80°C and salinity of 0-15% (w/v) and synthesize biosurfactant by using crude oil as sole carbon source under aerobic or anaerobic conditions. The yields of biosurfactant were ≈ 9.8 g/L and ≈ 2.8 g/L, respectively. Compositional analysis revealed that the fractionated components and compositions of the purified biosurfactant differed between aerobic (glycosides ≈ 50.3%, lipids ≈ 34.5%, peptide ≈ 15.2%, w/w) and anaerobic (glycosides ≈ 53.8%, lipids ≈ 31.2%, peptide ≈ 26.0%, w/w) conditions. The critical micelle concentrations of aerobic and anaerobic biosurfactant were 0.016 g/L and 0.022 g/L, respectively. Gas chromatography analysis indicated that strain H9 had a preference for utilizing medium- and long-length alkanes (C23-C43) under aerobic conditions, and degrading long alkanes (C33-C43) under anaerobic conditions. Physical simulation results showed that strain H9 and its biosurfactant have great potential use in microbial enhanced oil recovery, especially in high temperature and salinity oil reservoirs.
- Publication
Annals of Microbiology, 2012, Vol 62, Issue 4, p1779
- ISSN
1590-4261
- Publication type
Article
- DOI
10.1007/s13213-012-0436-5