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- Title
DNase-capture reveals differential transcription factor binding modalities.
- Authors
Kang, Daniel; Sherwood, Richard; Barkal, Amira; Hashimoto, Tatsunori; Engstrom, Logan; Gifford, David
- Abstract
We describe DNase-capture, an assay that increases the analytical resolution of DNase-seq by focusing its sequencing phase on selected genomic regions. We introduce a new method to compensate for capture bias called BaseNormal that allows for accurate recovery of transcription factor protection profiles from DNase-capture data. We show that these normalized data allow for nuanced detection of transcription factor binding heterogeneity with as few as dozens of sites.
- Subjects
DNA-binding proteins; DEOXYRIBONUCLEASES; NUCLEOTIDE sequencing; COMPUTATIONAL biology; GENOMICS
- Publication
PLoS ONE, 2017, Vol 12, Issue 12, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0187046