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- Title
Anti-Allergic and Anti-inflammatory Effects of Hydrosol Extracted from Zanthoxylum schinifolium Branch.
- Authors
Si Young Ha; Ji Young Jung; Dong Hwan Lee; Jae-Kyung Yang
- Abstract
Zanthoxylum schinifolium Sieb. et Zucc. (syn. Fagara schinifolia Engler) was studied for its potential anti-inflammatory properties. The hydrosol extract prepared from the Z. schinifolium branch was analyzed by gas chromatography/mass spectrometry. Here, five main chemical components were identified in the hydrosol of the branches of this shrub. The main chemical compounds in the branch inhibited both an Immunoglobulin E (IgE)-antigen complex and a dinitrophenyl-bovine serum albumin (DNP-BSA)-induced ß-hexosaminidase release in a dosedependent manner in RBL-2H3 mast cells, and at the tested concentrations did not show cytotoxicity to RBL-2H3 cells. Moreover, hydrosol obtained from the branch substantially inhibited a lipopolysaccharide (LPS) induced overproduction of intracellular active oxygen (ROS) and nitric oxide (NO). Consistently, the soluble Nethylmaleimide-sensitive factor-attachment protein receptor (SNARE) proteins of SNAP23, syntaxin4, VAMP7, and VAMP8 were remarkably decreased through hydrosol treatment. Hydrosol suppressed the activation of SNARE proteins in DNP-BSA-stimulated RBL-2H3 cells and inhibited ROS and NO in LPS-stimulated RAW264.7 cells. Camphor and estragole are the main chemical components of hydrosol and downregulate the LPS-induced phosphorylation of the SNARE proteins. The hydrosol obtained from the branch of Z. schinifolium has therapeutic benefits for allergic inflammatory diseases.
- Subjects
SNARE proteins; ZANTHOXYLUM; PROTEIN receptors; MAST cells; IMMUNOGLOBULIN E; MASS spectrometry
- Publication
BioResources, 2021, Vol 16, Issue 3, p5721
- ISSN
1930-2126
- Publication type
Article
- DOI
10.15376/biores.16.3.5721-5732