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- Title
Development of a semi-selective medium for isolation of Xanthomonas campestris pv. musacearum from banana plants.
- Authors
Leena Tripathi; Jaindra Tripathi; Wilberforce Tushemereirwe; Ranajit Bandyopadhyay
- Abstract
Abstract??BananaXanthomonaswilt, caused byXanthomonas campestrispv.musacearum, is a new threat to banana cultivation in eastern Africa. The causal bacterium grows slowly in culture and is easily overgrown by contaminants. A selective culture medium for isolation ofX. c.pv.musacearumwill facilitate disease study. A medium that suppressed saprophytic growth and possessed diagnostic characters for the pathogen was developed. Various carbon sources were tested with two isolates ofX. c.pv.musacearum, and sucrose was selected as main carbon source. The susceptibility ofX. c.pv. musacearumand other bacterial strains was tested with 29 different antibiotics. Cephalexin and cycloheximide had no effect onX. c.pv.musacearumbut cephalexin inhibited most of the saprophytes and cycloheximide inhibited the fungal contaminants. Based on these studies, we have developed a semi-selective medium YTSA-CC containing yeast extract (1%), tryptone (1%), sucrose (1%), agar (1.5%), cephalexin (50?mg?l?1) and cycloheximide (150?mg?l?1), pH 7.0. The pathogenX. c.pv.musacearumwas easily identified as yellowish, mucoid and circular colonies on YTSA-CC medium. This simple semi-selective medium was effective for isolation ofX. c.pv.musacearumfrom infected banana tissues and soil, and it should be a valuable tool in ecological and epidemiological studies.
- Subjects
XANTHOMONAS; PSEUDOMONADACEAE; BANANAS; MUSACEAE
- Publication
European Journal of Plant Pathology, 2007, Vol 117, Issue 2, p177
- ISSN
0929-1873
- Publication type
Article
- DOI
10.1007/s10658-006-9083-7