We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
In vitrodevelopment of mouse somatic nuclear transfer embryos: effects of donor cell passages and electrofusion.
- Authors
Gang Zhang; Qing-Yuan Sun; Da-Yuan Chen
- Abstract
SummaryIn this study, C57BL/6 adult male mouse ear fibroblast cells and Kunming mouse M2 oocytes were used as donors and recipients, respectively, to investigate the effect of passage number on donor cells and electrofusion times on the in vitrodevelopment of nuclear transfer (NT) embryos. The results demonstrated firstly that when the ear fibroblast cells from either 2?4, 5?7 or 8?10 passages were used as donors, respectively, to produce NT embryos, the number of passages undergone by the donor cells had no significant effect on the in vitrodevelopment of NT embryos. The developmental rates for morula/blastocyst were 15.2, 13.3 and 14.0%, respectively, which were not significantly difference (p> 0.05). Secondly, when the NT embryos were electrofused, there was no significant difference between the fusion ratio for the first electrofusion and the second electrofusion (p> 0.05). The developmental rates of the 2-cell and 4-cell stages that had undergone only one electrofusion, however, were significantly higher than those that had had two electrofusions (65.7% compared with 18.4% and 36.4% compared with 6.1%; p< 0.01), furthermore the NT embryos with two electrofusions could not develop beyond the 4-cell stage. This study suggests that this protocol might be an alternative method for mouse somatic cloning, even though electrofusion can exert negative effects on the development of NT embryos.
- Subjects
FERTILIZATION in vitro; TRANSPLANTATION of cell nuclei; SOMATIC cells; ELECTROFUSION; LABORATORY mice; BLASTOCYST
- Publication
Zygote, 2008, Vol 16, Issue 3, p223
- ISSN
0967-1994
- Publication type
Article
- DOI
10.1017/S096719940800467X