We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Characterization of the human heart mitochondrial proteome.
- Authors
Taylor, Steven W.; Fahy, Eoin; Zhang, Bing; Glenn, Gary M.; Warnock, Dale E.; Wiley, Sandra; Murphy, Anne N.; Gaucher, Sara P.; Capaldi, Roderick A.; Gibson, Bradford W.; Ghosh, Soumitra S.
- Abstract
To gain a better understanding of the critical role of mitochondria in cell function, we have compiled an extensive catalogue of the mitochondrial proteome using highly purified mitochondria from normal human heart tissue. Sucrose gradient centrifugation was employed to partially resolve protein complexes whose individual protein components were separated by one-dimensional PAGE. Total in-gel processing and subsequent detection by mass spectrometry and rigorous bioinformatic analysis yielded a total of 615 distinct protein identifications. All protein pl values, molecular weight ranges, and hydrophobicities were represented. The coverage of the known subunits of the oxidative phosphorylation machinery within the inner mitochondrial membrane was > 90%. A significant proportion of identified proteins are involved in signaling, RNA, DNA, and protein synthesis, ion transport, and lipid metabolism. The biochemical roles of 19% of the identified proteins have not been defined. This database of proteins provides a comprehensive resource for the discovery of novel mitochondrial functions and pathways.
- Subjects
MITOCHONDRIA; PROTEOMICS; HEART
- Publication
Nature Biotechnology, 2003, Vol 21, Issue 3, p281
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt793