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- Title
The Effectiveness of Oxysept® 1- Step Contact Lens Disinfecting Solution against Clinical Isolates of Acanthamoeba Cysts.
- Authors
Ghani, Mohamed Kamel Abd; Singh, Kirat Kaur Santokh; Nordin, Anisah; Suboh, Yusof; Rahim, Noraina Ab; Ahmad, Norazah
- Abstract
Introduction: Majority of individuals who acquire Acanthamoeba keratitis are contact lens wearers. A number of disinfection solutions are completely ineffective against Acanthamoeba, especially in its cysts form. Acanthamoeba keratitis could be prevented by the adequate use of an effective disinfection system. Objectives: This study investigates the effectiveness of Oxysept® 1-Step lens disinfecting solution against 4 clinical isolates of Acanthamoeba sp., based on the soaking time recommended by the manufacturer and soaking times of 4 hours, 6 hours and 8 hours. Materials and Methods: Cyst suspensions were prepared using Acanthamoeba cysts grown on Non-Nutrient Agar (NNA) for 10 days at 30°C (± 2°C). Oxysept® 1-Step contact lens disinfecting solution was used to determine its effectiveness as anti-Acanthamoeba agent. Cyst suspensions of each isolate were tested based on the manufacturers' recommended soaking time, 4 hours, 6 hours and 8 hours respectively. After the soaking time, 100 pi cyst suspension of each isolate was cultured onto NNA plates seeded with E. coli at 30°C (± 2°C) and observed daily for 14 days under an inverted microscope to detect the presence of trophozoites. Results: Oxysept® 1- Step was successful at inactivating the cysts of all the Acanthamoeba spp. isolates tested within all the testing times. Oxysept® 1- Step showed high anti-Acanthamoeba activity. Conclusion: Contact lens disinfecting solutions using hydrogen peroxide systems are probably the best choice for contact lens wearers in preventing Acanthamoeba keratitis.
- Subjects
MICROBIAL contamination; DISINFECTION &; disinfectants; ESCHERICHIA coli; ACANTHAMOEBA keratitis; MICROSCOPY; OPHTHALMIC drugs; PROTOZOA; TIME; PHARMACODYNAMICS; PREVENTION
- Publication
International Medical Journal, 2019, Vol 26, Issue 3, p196
- ISSN
1341-2051
- Publication type
Article