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- Title
Molecular Detection of Rifampicin Resistance in Mycobacterium tuberculosis: An Urban Study.
- Authors
Hussin, Salasawati; Das, Srijit; Yew Lok Woo; Nordin, Farizal; Zakil, Farizan; Razak, Hadi; Jamaludin, Norhayati; Ismail, Nordiana; Ghulam, Noor Hamiza; Nordin, Ainilhayati; Salleh, Sharifah Azura
- Abstract
Objective: The detection of multi-drug resistant tuberculosis (MDR-TB) by broth method requires eight weeks. Molecular technique shortens the duration and it is able to detect the resistant gene. The objective of this study was to determine the feasibility of using molecular technique to detect MDK-TB compared to broth method. Materials and methods: Samples were collected from 726 patients suspected to have tuberculosis throughout the year 2008 in Universiti Kebangsaan Malaysia Medical Centre (UKMMC). Growth on Lowenstein-Jensen media were confirmed as Mycobacteriuin tuberculosis using BDProbeTec™ ET Mycobacterium and susceptibility testing was performed using BACTEC™ MGIT™ 960. The 41 lbp fragment of rpo B gene was amplified and sequenced. Results: Fifty of the 726 samples (6.89%) grew Mycobacteriuin tuberculosis. MDR was detected in four of the 50 (8%) isolates of Mycobacteriuin tuberculosis. The sensitivity of molecular technique in detecting MDR- TB was 25% with a specificity of 100%. The positive predictive value was 100% whereas negative predictive value was 89.66%. Conclusion: Molecular technique was not an effective screening test for MDR-TB due to the low sensitivity.
- Subjects
MALAYSIA; TUBERCULOSIS diagnosis; TUBERCULOSIS risk factors; ACADEMIC medical centers; BIOPHYSICS; CHI-squared test; COMPUTER software; CULTURE media (Biology); DRUG resistance in microorganisms; GENETIC techniques; RESEARCH methodology; METROPOLITAN areas; MICROBIAL sensitivity tests; POLYMERASE chain reaction; RESEARCH funding; RIFAMPIN; TUBERCULOSIS; DATA analysis
- Publication
International Medical Journal, 2011, Vol 18, Issue 1, p63
- ISSN
1341-2051
- Publication type
Article