We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Vaginal Microbiome Components as Correlates of Cervical Human Papillomavirus Infection.
- Authors
Morales, Julia Andrade Pessoa; Marconi, Camila; El-Zein, Mariam; Ravel, Jacques; Pinto, Gabriel Victor da Silva; Silveira, Rosana; Lima, Moises Diogode; Carvalho, Newton Sergio de; Alves, Rosane Ribeiro Figueiredo; Parada, Cristina Maria Garcia de Lima; Leite, Sandra Helena Morais; Villa, Luisa L; Franco, Eduardo L; Silva, Marcia Guimarães da; Andrade Pessoa Morales, Julia; da Silva Pinto, Gabriel Victor; de Carvalho, Newton Sergio; de Lima Parada, Cristina Maria Garcia; da Silva, Marcia Guimarães
- Abstract
<bold>Background: </bold>Interplay between vaginal microbiome and human papillomavirus (HPV) remains unclear, partly due to heterogeneity of microbiota.<bold>Methods: </bold>We used data from 546 women enrolled in a cross-sectional study in 5 Brazil. We genotyped vaginal samples for HPV and sequenced V3-V4 region of 16S rRNA gene for vaginal microbiome analysis. We used stepwise logistic regression to construct 2 linear scores to predict high-risk HPV (hrHPV) positivity: one based exclusively on presence of individual bacterial taxa (microbiome-based [MB] score) and the other exclusively on participants' sociodemographic, behavioral, and clinical (SBC) characteristics. MB score combined coefficients of 30 (of 116) species. SBC score retained 6 of 25 candidate variables. We constructed receiver operating characteristic curves for scores as hrHPV correlates and compared areas under the curve (AUC) and 95% confidence intervals (CI).<bold>Results: </bold>Overall, prevalence of hrHPV was 15.8%, and 26.2% had a Lactobacillus-depleted microbiome. AUCs were 0.8022 (95% CI, .7517-.8527) for MB score and 0.7027 (95% CI, .6419-.7636) for SBC score (P = .0163).<bold>Conclusions: </bold>The proposed MB score is strongly correlated with hrHPV positivity-exceeding the predictive value of behavioral variables-suggesting its potential as an indicator of infection and possible value for clinical risk stratification.
- Subjects
BRAZIL; PAPILLOMAVIRUS diseases; RECEIVER operating characteristic curves; HUMAN microbiota; PAPILLOMAVIRUSES; VERTEBRATES; MICROBIOLOGY; PHENOMENOLOGICAL biology; CROSS-sectional method; RNA; VAGINA; VIRUS diseases; RESEARCH funding; CERVIX uteri tumors
- Publication
Journal of Infectious Diseases, 2022, Vol 226, Issue 6, p1084
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jiab547