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- Title
The clock protein period 2 synchronizes mitotic expansion and decidual transformation of human endometrial stromal cells.
- Authors
Muter, Joanne; Lucas, Emma S.; Yi-Wah Chan; Brighton, Paul J.; Moore, Jonathan D.; Lacey, Lauren; Quenby, Siobhan; Lam, Eric W.-F.; Brosens, Jan J.
- Abstract
Implantation requires coordinated interactions between the conceptus and surrounding decidual cells, but the involvement of clock genes in this process is incompletely understood. Circadian oscillations are predicated on transcriptional-translational feedback loops, which balance the activities of the transcriptional activators CLOCK (circadian locomotor output cycles kaput) and brain muscle arnt-like 1 and repressors encoded by PER (Period) and Cryptochrome genes. We show that loss of PER2 expression silences circadian oscillations in decidualizing human endometrial stromal cells (HESCs). Down-regulation occurred between 12 and 24 hours following differentiation and coincided with reduced CLOCK binding to a noncanonical E-box enhancer in the PER2 promoter. RNA sequencing revealed that premature inhibition of PER2 by small interfering RNA knockdown leads to a grossly disorganized decidual response. Gene ontology analysis highlighted a preponderance of cell cycle regulators among the 1121 genes perturbed upon PER2 knockdown. Congruently, PER2 inhibition abrogated mitotic expansion of differentiating HESCs by inducing cell cycle block at G2/M. Analysis of 70 midluteal endometrial biopsies revealed an inverse correlation between PER2 transcript levels and the number of miscarriages in women suffering reproductive failure (Spearman rank test, p = 20.3260; P= 0.0046). Thus, PER2 synchronizes endometrial proliferation with initiation of aperiodic decidual gene expression; uncoupling of these events may cause recurrent pregnancy loss.
- Subjects
STROMAL cells; CONNECTIVE tissue cells; MESENCHYMAL stem cells; ENDOMETRIUM; MISCARRIAGE
- Publication
FASEB Journal, 2015, Vol 29, Issue 4, p1603
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.14-267195