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- Title
Transformation of somatic cells into stem cell-like cells under a stromal niche.
- Authors
Seung Tae Lee; Seung Pyo Gong; Kyung Eun Yum; Eun Ju Lee; Chae Hyun Lee; Jun Hee Choi; Dae Yong Kim; Hojae Han; Kye-Seong Kim; Hysolli, Eriona; Ji Yeon Ahn; In-Hyun Park; Jae Yong Han; Jae-Wook Jeong; Jeong Mook Lim
- Abstract
To study the genomic plasticity of somatic cells without ectopic genetic manipulation, we cultured mouse fibroblasts with ovarian cells, embryonic fibroblasts of different strains, and parthenogenetic embryonic stem cells (ESCs). Of 41 trials, cell aggregation resembling nascent ESC colony from inner cell mass was detected in 9 cases (22%), and 6 cases (67%) yielded fibroblast-derived colonies with ESC morphology. Cells used in coculture provided the critical (P=0.0061) inducing factor for the aggregation. These colony-forming fibroblasts (CFFs) showed similar characteristics to those in ESCs and induced pluripotent stem cells (iPSCs), including pluripotency gene expression, in vitro differentiation, and teratoma formation. Furthermore, CFFs produced somatic chimera, although none showed germline chimerism. CFFs had a tetraploid-like karyotype, and their imprinting patterns differed from parthenogenetic ESCs, thereby confirming their nongermline transmissibility. We observed dysregulation of cell cycle-related proteins, as well as both homologous and heterologous recombination of genomic single-nucleotide polymorphisms in CFFs. Our observations provide information on somatic cell plasticity, resulting in stemness or tumorigenesis, regardless of colony-forming cell progenitors in the fibroblast population. The plasticity of somatic genomes under environmental influences, as well as acquisition of pluripotency by cell fusion, is also implicated.
- Subjects
MATERIAL plasticity; FIBROBLASTS; CYTOLOGICAL research; SOMATIC cells; CELLS
- Publication
FASEB Journal, 2013, Vol 27, Issue 7, p2644
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.12-223065