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- Title
Primocolonization is associated with colonic epithelial maturation during conventionalization.
- Authors
Tomas, Julie; Wrzosek, Laura; Bouznad, Nassim; Bouet, Stephan; Mayeur, Camille; Noordine, Marie-Louise; Honvo-Houeto, Edith; Langella, Philippe; Thomas, Muriel; Cherbuy, Claire
- Abstract
Interaction between the gut microbiota and the host starts immediately after birth with the progressive colonization of the sterile intestine. Our aim was to investigate die interactions taking place in the colonic epidielium after the first exposure to gut microbiota. Germ-free (GF) rats were inoculated with two different microbiotas: the first, obtained from suckling rats, was rich in primocolonizing bacteria and the second, obtained from adult rats, was representative of a mature microbiota. Once transferred into GF rats, these two microbiotas evolved such that they converged, and recapitulated die primocolonization pattern, mimicking the chronological scheme of implantation following birth. The two microbiotas induced common responses in die colonic epithelium: a transitory proliferative phase followed by a compensatory phase characterized by increases hi die abundance of p21Cip1 and p27Kip1 and in die number of goblet cells. The effects of die two microbiotas diverged only through their effects on colonic transporters. Analyses of solute carriers and aquaporins revealed that functional maturation was more pronounced following exposure to adult microbiota than suckling microbiota. The colon matured in parallel with the evolution of the microbiota composition, and we therefore suggest a link between intestinal events regulating homeostasis of the colon and modulation of microbial composition.
- Subjects
INTESTINES; CELL proliferation; BACTERIA; EPITHELIUM; HOMEOSTASIS
- Publication
FASEB Journal, 2013, Vol 27, Issue 2, p645
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.12-216861