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- Title
A cost-effective and efficient approach for generating and assembling reagents for conducting real-time PCR.
- Authors
Mote, Ridim D; Laxmikant, V Shinde; Singh, Surya Bansi; Tiwari, Mahak; Singh, Hemant; Srivastava, Juhi; Tripathi, Vidisha; Seshadri, Vasudevan; Majumdar, Amitabha; Subramanyam, Deepa
- Abstract
Real-time PCR is a widely used technique for quantification of gene expression. However, commercially available kits for real-time PCR are very expensive. The ongoing coronavirus pandemic has severely hampered the economy in a number of developing countries, resulting in a reduction in available research funding. The fallout of this will result in limiting educational institutes and small enterprises from using cutting edge biological techniques such as real-time PCR. Here, we report a cost-effective approach for preparing and assembling cDNA synthesis and real-time PCR mastermixes with similar efficiencies as commercially available kits. Our results thus demonstrate an alternative to commercially available kits.
- Subjects
COVID-19 pandemic; MOUSE leukemia viruses; DEVELOPING countries; REVERSE transcriptase; COMPLEMENTARY DNA
- Publication
Journal of Biosciences, 2021, Vol 47, Issue 1, p1
- ISSN
0250-5991
- Publication type
Article
- DOI
10.1007/s12038-021-00231-w