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- Title
Erythrocytic protein kinase C activity in primary hypertension.
- Authors
Ek; Toomik; Eriksson; Frithz; Ronquist; Engström; Ek; Ek, P; Toomik, R; Eriksson, S; Frithz, G; Ronquist, G; Engström, L
- Abstract
<bold>Objectives: </bold>Increased protein kinase C activity has been reported in erythrocytes from patients with primary hypertension and also from hypertensive rats. In this phenomenological study, we investigated whether a possible increased activity was the result of an augmented amount of enzyme molecules or a more active enzyme.<bold>Design: </bold>Collect blood samples, separate erythrocytes from other blood cells. After partial purification of protein kinase C in the erythrocyte lysate, assay the enzyme activity under optimal conditions using a specific peptide substrate.<bold>Setting: </bold>Central Hospital in Eskilstuna and University Hospital in Uppsala, Sweden.<bold>Subjects: </bold>Healthy individuals: 47 persons (20 women and 27 men). Ten patients with untreated primary hypertension.<bold>Main Outcome Measures: </bold>Erythrocytes were separated from leucocytes and platelets by passing through a cellulose column followed by repeated washings. Some proteins in the erythrocyte lysate interfering with protein kinase C assay were removed by chromatography on DEAE-cellulose.<bold>Results: </bold>The mean protein kinase C activity in erythrocytes from healthy individuals was 0.18 +/- 0.02 pmol [32P]phosphate min(-1) x 10(6) cells, regardless of sex and age. The corresponding value for patients with primary hypertension was 0.16 +/- 0.04 pmol [32P]phosphate min(-1) x 10(6) cells.<bold>Conclusions: </bold>The amount of protein kinase C, measured as the activity at optimal assay conditions, in erythrocytes from patients with primary hypertension is not critical for the development of moderate hypertension.
- Subjects
PROTEIN kinase C; ESSENTIAL hypertension; ERYTHROCYTES; BIOCHEMISTRY; BLOOD platelets; COMPARATIVE studies; HYPERTENSION; PHENOMENOLOGY; RESEARCH methodology; MEDICAL cooperation; RESEARCH; TRANSFERASES; EVALUATION research
- Publication
Journal of Internal Medicine, 1998, Vol 243, Issue 4, p299
- ISSN
0954-6820
- Publication type
journal article
- DOI
10.1046/j.1365-2796.1998.00255.x