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- Title
Human Regulatory CD8<sup>+</sup> T Cells.
- Authors
Ablamunits, Vitaly; Bisikirska, Brygida C.; Herold, Kevan C.
- Abstract
Administration of a humanized monoclonal anti-CD3 antibody (mAb) to patients with type 1 diabetes (T1D) increases their C-peptide responses and the CD8/CD4 ratio. Incubation of human peripheral blood mononuclear cells (PBMC) with mAb in vitro has been shown to induce CD8+ regulatory T cells (Tregs) capable of inhibiting proliferation of CD4+ T cells. We hypothesized that CD8+ Tregs function through secretion of cytokines. To test that possibility, we generated CD8+ Tregs, sorted them by FACS, incubated them with syngeneic CD8-depleted PBMC in the presence of staphylococcal enterotoxin B (SEB), and measured proliferation of T cells and cytokines. Using neutralizing anti-cytokine mAbs, we show that the inhibitory effect of CD8+ Tregs could be partially alleviated by anti-CCL-4, anti-TNF, and to a lesser extent anti-IL2, suggesting that these cytokines contribute to CD8+ Treg function.
- Subjects
MONOCLONAL antibodies; DIABETES; T cells; PEPTIDES; STAPHYLOCOCCAL diseases; CYTOKINES; CELL proliferation; AUTOIMMUNITY; MEDICAL research
- Publication
Annals of the New York Academy of Sciences, 2008, Vol 1150, p234
- ISSN
0077-8923
- Publication type
Article
- DOI
10.1196/annals.1447.000