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- Title
DNA-Binding Properties of Amidine-Substituted Spiropyran Photoswitches.
- Authors
Hammarson, Martin; Nilsson, Jesper R.; Li, Shiming; Lincoln, Per; Andréasson, Joakim
- Abstract
Two amidine-substituted spiropyran derivatives have been characterized with respect to the DNA-binding properties over a broad pH interval. The two derivatives differ in the number of positive charges. By varying the pH, the protonation state of the derivatives is also changed, allowing for additional variations in the charge distribution. We show that the closed spiro isomer does not bind for either of the two derivatives, whereas the open merocyanine forms bind both in the protonated and in the nonprotonated state, but with dramatically different binding constants. Flow-oriented linear dichroism (LD) measurements also show that there are differences in the binding modes between the various forms. We rationalize these differences in terms of structure and charge distribution.
- Subjects
SPIROPYRANS; DNA-binding proteins; AMIDINES; LINEAR dichroism; AMPLIFIED fragment length polymorphism
- Publication
Chemistry - A European Journal, 2014, Vol 20, Issue 48, p15855
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.201405113