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- Title
K4 channels underlie A-currents with highly variable inactivation time courses but homogeneous other gating properties in the nucleus tractus solitarii.
- Authors
Strube, Caroline; Saliba, Layal; Moubarak, Estelle; Penalba, Virginie; Martin-Eauclaire, Marie-France; Tell, Fabien; Clerc, Nadine
- Abstract
In the nucleus of the tractus solitarii (NTS), a large proportion of neurones express transient A-type potassium currents ( I) having deep influence on the fidelity of the synaptic transmission of the visceral primary afferent inputs to second-order neurones. Up to now, the strong impact of I within the NTS was considered to result exclusively from its variation in amplitude, and its molecular correlate(s) remained unknown. In order to identify which K channels underlie I in NTS neurones, the gating properties and the pharmacology of this current were determined using whole cell patch clamp recordings in slices. Complementary information was brought by immunohistochemistry. Strikingly, two neurone subpopulations characterized by fast or slow inactivation time courses (respectively about 50 and 200 ms) were discriminated. Both characteristics matched those of the K4 channel subfamily. The other gating properties, also matching the K4 channel ones, were homogeneous through the NTS. The activation and inactivation occurred at membrane potentials around the threshold for generating action potentials, and the time course of recovery from inactivation was rapid. Pharmacologically, I in NTS neurones was found to be resistant to tetraethylammonium (TEA), sea anemone toxin blood-depressing substance (BDS) and dendrotoxin (DTX), whereas Androctonus mauretanicus mauretanicus toxin 3 (AmmTX3), a scorpion toxin of the α-KTX 15 family that has been shown to block all the members of the K4 family, inhibited 80 % of I irrespectively of its inactivation time course. Finally, immunohistochemistry data suggested that, among the K4 channel subfamily, K4.3 is the prevalent subunit expressed in the NTS.
- Subjects
POTASSIUM channels; HOMOGENEOUS plasma; SOLITARY nucleus; NEURAL circuitry; NEURAL transmission; PHARMACOLOGY; IMMUNOHISTOCHEMISTRY; PHYSIOLOGY
- Publication
Pflügers Archiv: European Journal of Physiology, 2015, Vol 467, Issue 4, p789
- ISSN
0031-6768
- Publication type
Article
- DOI
10.1007/s00424-014-1533-z