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- Title
HPLC and Anti-Inflammatory Studies of the Flavonoid Rich Chloroform Extract Fraction of Orthosiphon Stamineus Leaves.
- Authors
Mun Fei Yam; Vuanghao Lim; Salman, Ibrahim Muhammad; Ameer, Omar Ziad; Lee Fung Ang; Rosidah, Noersal; Abdulkarim, Muthanna Fawzy; Abdullah, Ghassan Zuhair; Rusliza Basir; Amirin Sadikun; Mohd Zaini Asmawi
- Abstract
The aim of the present study was to verify the anti-inflammatory activity of Orthosiphon stamineus leaf extracts and to identify the active compound(s) contributing to its anti-inflammatory activity using a developed HPLC method. Active chloroform extract of O. stamineus was fractionated into three fractions using a dry flash column chromatography method. These three fractions were investigated for anti-peritoneal capillary permeability, in vitro nitric oxide scavenging activity, anti-inflammatory and nitric oxide (NO) inhibition using carrageenan-induced hind paw edema method. The flavonoid rich chloroform extract fraction (CF2) [containing sinensetin (2.86% w/w), eupatorin (5.05% w/w) and 3′-hydroxy-5,6,7,4′-tetramethoxyflavone (1.101% w/w)], significantly reduced rat hind paw edema, NO and decreased dye leakage to peritoneal cavity at p < 0.05. IC50 of in vitro NO scavenging of CF2 was 0.3 mg/mL. These results suggest that the anti-inflammatory properties of these CF2 may possibly be due to the presence of flavonoid compounds capable of affecting the NO pathway.
- Subjects
HIGH performance liquid chromatography; FLAVONOIDS; CHLOROFORM; NITRIC oxide; EDEMA
- Publication
Molecules, 2010, Vol 15, Issue 6, p4452
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules15064452