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- Title
Genome-Wide Characterization of Cytokinin Response Regulator in Grape and Expression Analyses during Berry Set Process.
- Authors
Bian, L.; Shi, B. X.; Yu, K. K; Guo, D. L.; Ji, X. R.; Ni, P. Y.; Yang, Y. J.; Zhang, G. H.; Yu, Y. H.
- Abstract
Cytokinin response regulators (RRs) are a family of transcription factors, which function as a signal output of the cytokinin two-component signaling pathway, play an essential role in plant growth and development. They have been identified in many plants, but little is known in grape. Also, how RRs meditated cytokinin signal pathway in grape berry set process is unclear. In this study, employing the tools of bioinformatics we identified the 32 genes of RRs in grape (Vitis vinifera), which distributed on 14 chromosomes, and they were divided into three group based on phylogenic analysis and conserved domain, including 14 A-type, 11 B-type and 7 pseudo-type RRs. We present a complete studied of response regulators gene family in grape, phylogenetic analysis, chromosomal location, conserved motif organization, gene structure. The expression of 32 VviRRs in control and N-(2-chloro-4-pyridyl)-N'-phenylurea CPPU treatment was explored using RNA-seq data, 9 VviRRs differential genes (DEGs) were selected for quantitative real-time PCR (qRT-PCR) verification after CPPU treatment. Our results provided a foundation for better understanding the mechanism of cytokinin mediated berry set which will be beneficial to make use of synthetic plant growth regulator in cultivation practice.
- Subjects
VITIS vinifera; BERRIES; GRAPES; PLANT regulators; REGULATOR genes; GENE families; PLANT growth
- Publication
Russian Journal of Plant Physiology, 2022, Vol 69, Issue 3, p1
- ISSN
1021-4437
- Publication type
Article
- DOI
10.1134/S1021443722030049