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- Title
Ion-exchange properties of the cell wall of reindeer lichen Cladonia rangiferina.
- Authors
Meychik, N. R.; Lyubimova, E. G.; Yermakov, I. P.
- Abstract
Ion-exchange properties of cell walls were investigated in reindeer lichen Cladonia rangiferina (L.) F. H. Wigg. In order to isolate cell walls, we used living parts of podetia as well as young parts (four upper internodes of podetia) and old parts (from the 4th to the 8th internode). We studied functional dependences of cell wall ion-exchange capacity on pH in the range from 2 to 12 and constant ionic strength of solution equal to 10 mM. It was found that three-dimensional structure of C. rangiferina cell walls comprised three types of ionogenic groups, which determine ion-exchange properties of the cell walls. They are amino groups with pKa of about 3, carboxyl groups with pKa of about 7, and phenolic OH-groups with pKa of about 10. The content of groups of each type and their ionization constants were determined, and it was shown that, in the cell walls of young parts, the content of amino groups and carboxylic groups was greater than in old parts of podetia (by 1.5 and 2.0 times, respectively). It was found that with age the content of nitrogen and the proportion of deacetylated amino groups in the cell walls changed from 34% (young parts of podetia) to 40% (old parts of podetia). It was shown that in C. rangiferina N-acetyl glucosamine and glucosamine are not the main monomers of cell wall polymers because both in thalli and in the cell walls isolated therefrom the content of total nitrogen was less than 1%.
- Subjects
CLADONIA; CELL membranes; ION exchange (Chemistry); AMINO group; GLUCOSAMINE
- Publication
Russian Journal of Plant Physiology, 2010, Vol 57, Issue 2, p260
- ISSN
1021-4437
- Publication type
Article
- DOI
10.1134/S1021443710020147