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- Title
Composite branched and linear F-actin maximize myosin-induced membrane shape changes in a biomimetic cell model.
- Authors
Sakamoto, Ryota; Murrell, Michael P.
- Abstract
The architecture of the actin cortex determines the generation and transmission of stresses, during key events from cell division to migration. However, its impact on myosin-induced cell shape changes remains unclear. Here, we reconstitute a minimal model of the actomyosin cortex with branched or linear F-actin architecture within giant unilamellar vesicles (GUVs, liposomes). Upon light activation of myosin, neither the branched nor linear F-actin architecture alone induces significant liposome shape changes. The branched F-actin network forms an integrated, membrane-bound "no-slip boundary" -like cortex that attenuates actomyosin contractility. By contrast, the linear F-actin network forms an unintegrated "slip boundary" -like cortex, where actin asters form without inducing membrane deformations. Notably, liposomes undergo significant deformations at an optimized balance of branched and linear F-actin networks. Our findings highlight the pivotal roles of branched F-actin in force transmission and linear F-actin in force generation to yield membrane shape changes. Investigating shape changes of GUVs with actomyosin cortex suggests that neither branched nor linear F-actin alone can induce the shape change; the combination of branched and linear F-actin is essential to yielding membrane shape changes.
- Subjects
F-actin; BIOMIMETIC materials; MYOSIN; CELL morphology; CELL migration; CELL division; ACTOMYOSIN
- Publication
Communications Biology, 2024, Vol 7, Issue 1, p1
- ISSN
2399-3642
- Publication type
Article
- DOI
10.1038/s42003-024-06528-4