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- Title
IN SILICO PROTEIN-INTERACTOME ANALYSIS AND GENE EXPRESSION PROFILING OF RICE G-PROTEIN COUPLED RECEPTOR-HOMOLOG.
- Authors
Srivastava, Gyan Prakash; Singh, Madhavi; Yadav, Dinesh Kumar
- Abstract
The classical G - protein coupled receptor (GPCR) is characterized as seven membrane-spanning protein with helical domains that interact with the intracellular heterotrimeric G-proteins to transmit signals. While the occurrence of GPCRs in animal is well documented, enough evidences support its presence in plants as well. The current research employs computational protein-interactome mapping of Oryza sativa GPCR homolog (OsGPCR), which reveals interaction with all the G-protein subunits (Gα, Gb, Gγ) and other key proteins (MLO and LFL1) analogous to the canonical GPCRs. The Gene Ontology analysis signifies that the OsGPCR-homolog posess evolutionary conserved signatures of a typical GPCR at the molecular, biological, as well as subcellular levels with promising score. Moreover, the tissue-specific expression profiling of OsGPCR performed for the nine seleted tissues of rice revealed least fluctuations in pistil (1.35 FPKM) with reference to house-keeping gene expression while the highest levels of expression was reported in anthers (32.32 FPKM). Nevertheless, OsGPCR expression was recorded in almost every tissue with the respective magnitude of 3.99, 5.32, 9.41, 9.98, 11.14, 12.69 and13.13 FPKM in callus, shoot, aleurone, seed, root, panicle and leaves. In sum, the conducted study strongly proposes that the OsGPCR-homolog has all the key biological, functional and evolutionary features of a canonical GPCR and exhibit a tissue-specific differential expression. Further, identification and annotation of the four uncharacterized proteins mapped through the interactome with relatively high scores will provide an insight into their functionality, thereby, highlighting their relevance to G - protein signaling pathways and also expanding the repertoire of plant G-protein signaling elements. Wet lab studies are required to validate the proposed expression profiles.
- Publication
Biochemical & Cellular Archives, 2023, Vol 23, Issue 1, p157
- ISSN
0972-5075
- Publication type
Article
- DOI
10.51470/bca.2023.23.1.157