We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Gene cloning, heterologous overexpression and optimized refolding of the NAD-glutamate dehydrogenase from Haloferax mediterranei.
- Authors
Díaz, Susana; Pérez-Pomares, Francisco; Pire, Carmen; Ferrer, Juan; Bonete, María-José
- Abstract
The NAD-dependent glutamate dehydrogenase (GDH) gene from the halophilic archaeon Haloferax mediterranei has been cloned. The analysis of the nucleotide sequence revealed an open reading frame of 1323 bp that encodes a NAD-GDH. The amino acid sequence displayed high homology with those from other sources, especially the highly conserved residues involved in 2-oxoglutarate binding. The expression of this gene in Escherichia coli, the refolding and further characterization, yielded a fully active NAD-GDH with the same features than those found for the wild-type enzyme. This halophilic NAD-GDH showed a highly dependence on salts for both stability and activity, being essential for the refolding of the recombinant enzyme.
- Subjects
HOMOLOGY (Biology); DEHYDROGENASES; NUCLEOTIDE sequence; AMINO acid sequence; ESCHERICHIA coli; RECOMBINANT blood proteins
- Publication
Extremophiles, 2006, Vol 10, Issue 2, p105
- ISSN
1431-0651
- Publication type
Article
- DOI
10.1007/s00792-005-0478-8