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- Title
超声辅助低共熔溶剂提取的鳞杯伞多糖的体外消 化与酵解特性.
- Authors
耿雪冉; 王无霞; 刘荣柱; 张德芳; 侯淑婷; 李泽辉; 葛广亮; 常明昌; 孟俊龙
- Abstract
This study aims at investigating the effects of in vitro digestion and fecal fermentation on the chemical composition, structural characteristics and biological activities of Clitocybe squamulosa polysaccharides (D-CSFP) extracted by ultrasound-assisted deep eutectic solvents. The results showed that after saliva, stomach and intestine digestion, the content of D-CSFP increased from 0.44 mg/mL to 0.45, 0.55 and 0.57 mg/mL respectively and the molecular weight of D-CSFP decreased from 35 312 Da to 32 090, 25 519 and 25 473 Da respectively, but the structure of D-CSFP had no obvious change. After fecal fermentation in vitro, the OD600 of D-CSFP fermentation broth increased first and then decreased (0.63→ 1.24→1.19); pH decreased from 6.75 to 6.13 (P<0.05); the total carbohydrate content in D-CSFP fermentation broth decreased from 8.59 mg/mL to 5.05 mg/mL (P<0.05); the total short chain fatty acids (SCFA) content was significantly increased from 1.83 mmol/L to 11.15 mmol/L (P<0.05). 16S rRNA sequencing results showed that D-CSFP could effectively promote the relative abundance of beneficial bacteria (such as Bacteroides, Prevotella and Dialister), and inhibit the propagation of harmful bacteria (such as Klebsiella). In conclusion, D-CSFP can be used as a potential functional food to promote intestinal health.
- Subjects
SHORT-chain fatty acids; MOLECULAR weights; BACTEROIDES; CHOLINE chloride; FERMENTATION; FUNCTIONAL foods; POLYSACCHARIDES; CALPROTECTIN
- Publication
Mycosystema, 2024, Vol 43, Issue 4, p1
- ISSN
1672-6472
- Publication type
Article
- DOI
10.13346/j.mycosystema.230272