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- Title
A network of Rab GTPases controls phagosome maturation and is modulated by Salmonella enterica serovar Typhimurium.
- Authors
Smith, Adam C.; Do Heo, Won; Braun, Virginie; Jiang, Xiuju; Macrae, Chloe; Casanova, James E.; Scidmore, Marci A.; Grinstein, Sergio; Meyer, Tobias; Brumell, John H.
- Abstract
Members of the Rab guanosine triphosphatase (GTPase) family are key regulators of membrane traffic. Here we examined the association of 48 Rabs with model phagosomes containing a non-invasive mutant of Salmonella enterica serovar Typhimurium (S. Typhimurium). This mutant traffics to lysosomes and allowed us to determine which Rabs localize to a maturing phagosome. In total, 18 Rabs associated with maturing phagosomes, each with its own kinetics of association. Dominant-negative mutants of Rab23 and 35 inhibited phagosome--lysosome fusion. A large number of Rab GTPases localized to wild-type Salmonella-containing vacuoles (SCVs), which do not fuse with lysosomes. However, some Rabs (8B, 13, 23, 32, and 35) were excluded from wild-type SCVs whereas others (5A, 5B, 5C, 7A, 11A, and 11B) were enriched on this compartment. Our studies demonstrate that a complex network of Rab GTPases controls endocytic progression to lysosomes and that this is modulated by S. Typhimurium to allow its intracellular growth.
- Subjects
GUANOSINE triphosphatase; SALMONELLA typhimurium; LYSOSOMES; CELL growth; PHOSPHATASES
- Publication
Journal of Cell Biology, 2007, Vol 176, Issue 3, p263
- ISSN
0021-9525
- Publication type
Article
- DOI
10.1083/jcb.200611056